Clinical application of multiplex quantitative fluorescent polymerase chain reaction (QF-PCR) for the rapid prenatal detection of common chromosome aneuploidies
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چکیده
منابع مشابه
Rapid Prenatal Diagnosis of Chromosomal Aneuploidies Using Quantitative Fluorescence Polymerase Chain Reaction (QF-PCR)
Introduction: Prenatal diagnosis plays an important role in determining the health of fetus, which is why rapid diagnostic tests are so important. One of these quick diagnostic tests is quantitative fluorescent-polymerase chain (QFPCR) technique. This technique employed to detect chromosomal aneuploidies (13, 18, 21, X and Y). In this method, there is no need for cell culturing. QFPCR is rapid,...
متن کاملQuantitative Fluorescence Polymerase Chain Reaction (QF-PCR) for Prenatal Diagnosis of Chromosomal Aneuploidies
Genomic aneuploidy is a common cause of human genetic disorders and cytogenetic analysis of metaphase karyotypes remain the standard method to identify aneuploidies and balanced translocations. Quantitative Fluorescence PCR (QF-PCR) is an alternative method in which DNA polymorphic markers on chromosomes, is used to determine the presence of different alleles. The assay based on the use of info...
متن کاملRapid prenatal diagnosis of common chromosome aneuploidies by QF-PCR. Assessment on 18,000 consecutive clinical samples.
The quantitative fluorescent PCR (QF-PCR) assay, introduced during the last few years, allows prenatal diagnoses of common chromosome aneuploidies in a few hours after sampling. We report the first assessment of QF-PCR performed on a large cohort of 18,000 consecutive clinical specimens analysed in two different Centres. All samples were analysed by QF-PCR using several selected STR markers tog...
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Prenatal diagnosis is testing for diseases or conditions in a fetus or embryo before it is born. It employs a variety of techniques to determine the health and condition of an unborn fetus. The main goal of this process is to perform prenatal diagnosis at the earliest possible stage of gestation. In this regard, quantitative fluorescent-polymerase chain reaction (QF-PCR), a novel technique that...
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During the past few years, rapid prenatal diagnosis of chromosome aneuploidies has been successfully achieved by quantitative fluorescent PCR (QF-PCR) amplification of chromosome-specific small tandem repeats (STR). This approach has proven to be very useful in clinical settings, since it allows the detection of major numerical disorders in a few hours after sampling. For the detection of Turne...
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ژورنال
عنوان ژورنال: Molecular Human Reproduction
سال: 2001
ISSN: 1460-2407
DOI: 10.1093/molehr/7.10.1001